basal media b5 Search Results


b5 basal medium  (PhytoTechnology Laboratories)
90
PhytoTechnology Laboratories b5 basal medium
Generation and identification of transgenic Hs1 pro-1 and cZR3 plants from hypocotyle explants of B. napus cv. Zheshuang 758 by A. tumefaciens mediated transformation. ( A , B ) Diagram of T-DNA region of the binary vector pAM194- Hs1 pro-1 ( A ) and pAM194- cZR3 ( B ). LB, T-DNA left border; RB, right border; P35S, Cauliflower mosaic virus (CaMV) 35S promoter; NOS, nopaline synthase terminator; MCS, multi clone site including Xhol restriction site; Hs1 pro-1 , Beta vulgaris Hs1 pro-1 gene open read fragment sequence; cZR3 , Beta vulgaris resistance gene sequence; GUS , β-glucuronidase report gene; NPTII , neomycin phosphotranferase gene for kanamycin resistance. ( C ) Callus induction from hypocotyl explants after co-cultivation with A. tumefaciens . ( D ) Shoots regenerated on SIM. ( E ) Shoots <t>elongation</t> on SEM. ( F ) Shoots rooted on RM. ( G , H ) PCR assay for transgenic Hs1 pro-1 plants ( G ) and cZR3 plants ( H ) M means 1 kb DNA ladder; P means Hs1 or cZR3 plasmid DNA, W means non-transgenic plant DNA; Hs1-1, Hs1-2 and Hs1-3 mean T0 independent transgenic Hs1 pro-1 plants; cZR3-1, cZR3-2 and cZR3-3 mean T0 independent transgenic cZR3 plants. ( I ) Southern blot assay for T0 independent transgenic cZR3 and Hs1 pro-1 lines, P1P2 means Hs1 and cZR3 plasmid DNA, respectively; 1, 3, and 5 mean T0 independent transgenic Hs1 plants; 2, 4, and 6 mean T0 independent transgenic cZR3 plants.
B5 Basal Medium, supplied by PhytoTechnology Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/basal+media+b5/gamborg%E2%80%99s+b5+medium/pmc06479909-155-9-19
Average 90 stars, based on 1 article reviews
b5 basal medium - by Bioz Stars, 2026-09
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msb5 media  (PhytoTechnology Laboratories)
90
PhytoTechnology Laboratories msb5 media
Generation and identification of transgenic Hs1 pro-1 and cZR3 plants from hypocotyle explants of B. napus cv. Zheshuang 758 by A. tumefaciens mediated transformation. ( A , B ) Diagram of T-DNA region of the binary vector pAM194- Hs1 pro-1 ( A ) and pAM194- cZR3 ( B ). LB, T-DNA left border; RB, right border; P35S, Cauliflower mosaic virus (CaMV) 35S promoter; NOS, nopaline synthase terminator; MCS, multi clone site including Xhol restriction site; Hs1 pro-1 , Beta vulgaris Hs1 pro-1 gene open read fragment sequence; cZR3 , Beta vulgaris resistance gene sequence; GUS , β-glucuronidase report gene; NPTII , neomycin phosphotranferase gene for kanamycin resistance. ( C ) Callus induction from hypocotyl explants after co-cultivation with A. tumefaciens . ( D ) Shoots regenerated on SIM. ( E ) Shoots <t>elongation</t> on SEM. ( F ) Shoots rooted on RM. ( G , H ) PCR assay for transgenic Hs1 pro-1 plants ( G ) and cZR3 plants ( H ) M means 1 kb DNA ladder; P means Hs1 or cZR3 plasmid DNA, W means non-transgenic plant DNA; Hs1-1, Hs1-2 and Hs1-3 mean T0 independent transgenic Hs1 pro-1 plants; cZR3-1, cZR3-2 and cZR3-3 mean T0 independent transgenic cZR3 plants. ( I ) Southern blot assay for T0 independent transgenic cZR3 and Hs1 pro-1 lines, P1P2 means Hs1 and cZR3 plasmid DNA, respectively; 1, 3, and 5 mean T0 independent transgenic Hs1 plants; 2, 4, and 6 mean T0 independent transgenic cZR3 plants.
Msb5 Media, supplied by PhytoTechnology Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/basal+media+b5/msb5+media/10__1016_slash_j__pmpp__2018__03__001-52-18-28
Average 90 stars, based on 1 article reviews
msb5 media - by Bioz Stars, 2026-09
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b5 (15) vitamins  (HiMedia Laboratories)
90
HiMedia Laboratories b5 (15) vitamins
Generation and identification of transgenic Hs1 pro-1 and cZR3 plants from hypocotyle explants of B. napus cv. Zheshuang 758 by A. tumefaciens mediated transformation. ( A , B ) Diagram of T-DNA region of the binary vector pAM194- Hs1 pro-1 ( A ) and pAM194- cZR3 ( B ). LB, T-DNA left border; RB, right border; P35S, Cauliflower mosaic virus (CaMV) 35S promoter; NOS, nopaline synthase terminator; MCS, multi clone site including Xhol restriction site; Hs1 pro-1 , Beta vulgaris Hs1 pro-1 gene open read fragment sequence; cZR3 , Beta vulgaris resistance gene sequence; GUS , β-glucuronidase report gene; NPTII , neomycin phosphotranferase gene for kanamycin resistance. ( C ) Callus induction from hypocotyl explants after co-cultivation with A. tumefaciens . ( D ) Shoots regenerated on SIM. ( E ) Shoots <t>elongation</t> on SEM. ( F ) Shoots rooted on RM. ( G , H ) PCR assay for transgenic Hs1 pro-1 plants ( G ) and cZR3 plants ( H ) M means 1 kb DNA ladder; P means Hs1 or cZR3 plasmid DNA, W means non-transgenic plant DNA; Hs1-1, Hs1-2 and Hs1-3 mean T0 independent transgenic Hs1 pro-1 plants; cZR3-1, cZR3-2 and cZR3-3 mean T0 independent transgenic cZR3 plants. ( I ) Southern blot assay for T0 independent transgenic cZR3 and Hs1 pro-1 lines, P1P2 means Hs1 and cZR3 plasmid DNA, respectively; 1, 3, and 5 mean T0 independent transgenic Hs1 plants; 2, 4, and 6 mean T0 independent transgenic cZR3 plants.
B5 (15) Vitamins, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/basal+media+b5/b5++15++vitamins/10__1007_slash_bf03321916-34-6-23
Average 90 stars, based on 1 article reviews
b5 (15) vitamins - by Bioz Stars, 2026-09
90/100 stars
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Generation and identification of transgenic Hs1 pro-1 and cZR3 plants from hypocotyle explants of B. napus cv. Zheshuang 758 by A. tumefaciens mediated transformation. ( A , B ) Diagram of T-DNA region of the binary vector pAM194- Hs1 pro-1 ( A ) and pAM194- cZR3 ( B ). LB, T-DNA left border; RB, right border; P35S, Cauliflower mosaic virus (CaMV) 35S promoter; NOS, nopaline synthase terminator; MCS, multi clone site including Xhol restriction site; Hs1 pro-1 , Beta vulgaris Hs1 pro-1 gene open read fragment sequence; cZR3 , Beta vulgaris resistance gene sequence; GUS , β-glucuronidase report gene; NPTII , neomycin phosphotranferase gene for kanamycin resistance. ( C ) Callus induction from hypocotyl explants after co-cultivation with A. tumefaciens . ( D ) Shoots regenerated on SIM. ( E ) Shoots elongation on SEM. ( F ) Shoots rooted on RM. ( G , H ) PCR assay for transgenic Hs1 pro-1 plants ( G ) and cZR3 plants ( H ) M means 1 kb DNA ladder; P means Hs1 or cZR3 plasmid DNA, W means non-transgenic plant DNA; Hs1-1, Hs1-2 and Hs1-3 mean T0 independent transgenic Hs1 pro-1 plants; cZR3-1, cZR3-2 and cZR3-3 mean T0 independent transgenic cZR3 plants. ( I ) Southern blot assay for T0 independent transgenic cZR3 and Hs1 pro-1 lines, P1P2 means Hs1 and cZR3 plasmid DNA, respectively; 1, 3, and 5 mean T0 independent transgenic Hs1 plants; 2, 4, and 6 mean T0 independent transgenic cZR3 plants.

Journal: International Journal of Molecular Sciences

Article Title: BvcZR3 and BvHs1 pro-1 Genes Pyramiding Enhanced Beet Cyst Nematode ( Heterodera schachtii Schm.) Resistance in Oilseed Rape ( Brassica napus L.)

doi: 10.3390/ijms20071740

Figure Lengend Snippet: Generation and identification of transgenic Hs1 pro-1 and cZR3 plants from hypocotyle explants of B. napus cv. Zheshuang 758 by A. tumefaciens mediated transformation. ( A , B ) Diagram of T-DNA region of the binary vector pAM194- Hs1 pro-1 ( A ) and pAM194- cZR3 ( B ). LB, T-DNA left border; RB, right border; P35S, Cauliflower mosaic virus (CaMV) 35S promoter; NOS, nopaline synthase terminator; MCS, multi clone site including Xhol restriction site; Hs1 pro-1 , Beta vulgaris Hs1 pro-1 gene open read fragment sequence; cZR3 , Beta vulgaris resistance gene sequence; GUS , β-glucuronidase report gene; NPTII , neomycin phosphotranferase gene for kanamycin resistance. ( C ) Callus induction from hypocotyl explants after co-cultivation with A. tumefaciens . ( D ) Shoots regenerated on SIM. ( E ) Shoots elongation on SEM. ( F ) Shoots rooted on RM. ( G , H ) PCR assay for transgenic Hs1 pro-1 plants ( G ) and cZR3 plants ( H ) M means 1 kb DNA ladder; P means Hs1 or cZR3 plasmid DNA, W means non-transgenic plant DNA; Hs1-1, Hs1-2 and Hs1-3 mean T0 independent transgenic Hs1 pro-1 plants; cZR3-1, cZR3-2 and cZR3-3 mean T0 independent transgenic cZR3 plants. ( I ) Southern blot assay for T0 independent transgenic cZR3 and Hs1 pro-1 lines, P1P2 means Hs1 and cZR3 plasmid DNA, respectively; 1, 3, and 5 mean T0 independent transgenic Hs1 plants; 2, 4, and 6 mean T0 independent transgenic cZR3 plants.

Article Snippet: The multiple induced shoots were isolated and transferred to shoot elongation media [ ] (SEM) (B5 basal medium from Phytotechnology laboratories ® 3.21 g/L, 10 g/L sucrose, 50 mg/L Kan, 9 g/L Agar, pH5.8).

Techniques: Transgenic Assay, Transformation Assay, Plasmid Preparation, Virus, Sequencing, Southern Blot